I'm a graduate student at the Department of Physiology and Biophysics at the University of Arkansas for Mediacal Sciences.

Some personal information can be found here.

Intracellular membrane trafficking: molecular mechanisms responsible for the generation and maintenance of intracellular membrane-bound compartments, mechanisms and machinery which maintain high specificity of vesicle tethering and docking.

Golgi vesicle tethering factors: role of the Conserved Oligomeric Golgi (COG) complex in the intra-Golgi retrograde membrane trafficking, the COG complex interaction with SNAREs, Rab-GTP and the COPI vesicle coat.

Protein glycosylation disorder: depletion of the COG complex which is accompanied by accumulation of COG complex dependent (CCD) vesicles, cargo of non-tethered CCD vesicles, production of underglycosylated proteins.

Ph.D. student at the University of Arkansas for Medical Sciences (UAMS), the USA.

Expected degree: Ph.D. in Physiology.

Shupyka L.P. Postgraduate Medical Academy of Ukraine.

Degree: M.S. in Microbiology and Virology.

Theses: "Factors affecting adsorption of rota- and enteroviruses on to smectites".

National Medical University (NMUU) of Ukraine, Kiev.

Major: preventive medicine.

Degree: specialist.

Goethe Institut, Kiev

Major: German Language

Degree: middle level diploma.

PhD research at the Department of Physiology, UAMS.

COG complex - mediated recycling of Golgi enzymes.

Intracellular membrane trafficking is mediated by a large variety of molecules. The COG complex is a tethering molecule, which resides on the cis-Golgi. Most likely it functions in tethering of transport intermediates to the proper Golgi compartments. Depletion of the COG complex ultimately leads to the development of defects in protein glycosylation.

Postgraduate research at the Department of Virology, NMUU.

Adsorption activity of natural smectites.

This research was aimed on the investigation of physical factors affecting adsorbtion activity of smectites. Rotaviral infection constitutes twenty percent of all newborn infectious diseases. Natural smectites (sorbents) adsorb viruses, thus eliminating viral infection.

Gathered and processed information pertinent such infectious diseases as botulism, measles and hepatitis B.

Teaching assistance in class "Epidemiology of viral diseases"

Microbiologist in the Center of Public Health.

Laboratory assistant at the Department of Epidemiology, NMUU (part time).

DNA and protein electrophoresis,

DNA cloning techniques,

Tissue culture cells handling,

Transfection of tissue culture with DNA and siRNA,

Organelle separation and analysis,

Immunofluorescence technique.

Travel award in Graduate student Division for the poster presentation at the UAMS Student Research Days.

Best scientific project award at the 57-th international student medical conference. Section of epidemiology and virology, Art. "Epidemiology analysis of botulism in Ukraine during 1991-2001 years"

Membership in the student scientific society of NMUU, Kiev, Ukraine.

The third place award at the 54-th international medical student conference, section of epidemiology and virology, theme "State epidemiology service in the USA"

The second place award in pharmacology competition, NMUU, Kiev, Ukraine

A. Shestakova, S. Zolov and V. Lupashin. COG complex-mediated recycling of Golgi glycosyltransferases is essential for normal protein glycosylation. In preparation.

A. Shestakova et al.. "Epidemiology analysis of botulism in Ukraine during 1991-2001 period", Materials of the 57-th international student medical conference, 2002, Section of epidemiology and virology, page 40.

A. Shestakova et al., "Epidemiology analysis of measles in Ukraine before introduction of mass immunization (1990-1999 period.)", Materials of the 55-th international student medical conference, 2000, Section of epidemiology and virology, page 12c.

A. Shestakova et al., State epidemiology service in the USA, Materials of the 54-th international student medical conference, 1999, Section of epidemiology and Virology, page 169.

Curriculum Vitae [PDF]

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Updated: September, 2005